Tanja is a visiting Professor from Florida Atlantic University. We’re collaborating on a project that investigates the role of G protein-coupled receptors in regulating neuronal activity which, according to Tanja, makes us finally do ‘proper mechanistic neuroscience’. Apart from sharing her expertise on sub-cellular signaling pathways, Tanja helps us push our fly game.
Fathima’s interest lies in studying the neural circuits underlying the plethora of behaviours displayed by animals. She is especially fascinated by how different complex behaviours are adapted to changes in the environment.
The fact that the basic units of the brain are separate, discrete cells was still debatable in the late nineteenth century, although vast advances had already been made in understanding many other physiological aspects of our body. What makes the brain so difficult to decipher is its anatomical and functional complexity. One approach to understanding the functional principles of the organ that determines who we are and how we perceive and interact with the world is to study numerically simpler brains, which are nonetheless capable of producing and controlling complex behaviours.
Currently, Fathima is studying the descending pathways that convey information about the environment from sensory organs and central brain circuits to lower motor centers in flies, with a focus on how these descending pathways control and execute locomotion. At present, she is building different setups and standardizing protocols that will allow her to optogenetically manipulate various descending neurons to study more closely the flexible control of locomotion in flies and she is in absolute awe of it. Once our protocols and setups are established, Fathima’s experiments will help us develop a better understanding of how descending pathways modify and direct the ongoing behaviour.
Fathima hails from the southern part of India. She holds a bachelor’s degree in Botany and Biotechnology from Mahatma Gandhi University, Kerala and a master’s degree in Biotechnology from the Cochin University of Science and Technology. As part of her master’s thesis, she worked on developing a new method to probe exclusively the post-ingestive nutrient sensing in flies using liposomes with Dr Gaurav Das, NCCS, Pune and Dr Sneha Bajpe, Symbiosis International, Pune. She continued the work at the NCCS with the same group before moving to Würzburg to embark on her PhD project.
P.S: Fathima considers reading literary fiction as her sport and was delighted to find out about the big library on the campus in Würzburg. Recently, she has also re-invented her interest in gaming.
One of the most complicated and intriguing objects known to humanity can be found right inside our own head: the human brain. Despite the fact that humans have been fascinated with their brain for centuries, even simple nervous systems, like those of a worm or a fly, remain beyond our grasp. In the last decades, a huge effort has been made to advance our knowledge about the nervous system, but we are still a long way from being able to claim that we ‘understand’ the brain. Studying the brain remains challenging because the experiments we can carry out with the available techniques focus either on single neurons with a high level of precision, or on larger networks and brain areas at the price of losing information about sub-threshold neuronal dynamics. Unfortunately, most of the functions of the nervous system arise from global network activity, but we cannot record all the neurons with high precision at the same time. In this situation, computational models come to our aid, allowing us to develop an insight into the properties of neural networks at a global level, which are difficult or impossible to measure with empirical approaches. These data can then be exploited to design better experiments and to create new, improved hypotheses about network functions.
Modulatory neurons are key to understanding complex neural networks since they enable flexible, adaptive processing of external sensory cues and internal state signals. Since the complexity of these systems is quite high, Federico’s goal is to develop a computational model to understand how the ensemble of neuromodulators acts together to mediate flexible sensorimotor processing and adjust the metabolism of flies to ever-changing external and internal demands. To this end, Feffo is combining patch-clamp recordings from modulatory neurons and sensorimotor pathways with Hodgkin-Huxley type modeling approaches.
Federico holds a Bachelor’s degree in Natural Sciences and a Master’s degree in Neurobiology from La Sapienza-University of Rome, Italy. He also attended a one-year master course at the Advanced School in Artificial Intelligence (AS-AI) held by the Institute of Cognitive Sciences and Technologies, National Research Council (CNR-ISTC) of Rome. Thus, he acquired the necessary skills to complete his MSc thesis in computational neuroscience under the supervision of Dr. Gianluca Baldassarre in the same institute (CNR-ISTC).
To ensure survival in an ever-changing, complex world, animal behavior needs to be flexible and adaptive. Nervous systems have evolved to enable behavioral responses to a wide variety of sensory stimuli, but the adequate behavioral response to a given stimulus is highly context-dependent, and behavioral or internal states accordingly affect sensorimotor processing. For example, locomotion modulates responses of visual neurons, and hunger increases food-searching behavior and shifts taste preferences. Despite their ubiquitous importance, the neural mechanisms enabling context-dependent sensorimotor flexibility are not well understood. My Emmy Noether research program ‘Neural mechanisms enabling context-dependent sensorimotor flexibility’ aims to discover fundamental principles of motor control, in particular with regard to sensorimotor flexibility, by leveraging the power of neurogenetics, electron microscopy-based circuit reconstruction, and in-vivo patch-clamp recordings in behaving Drosophila.
The Ache Lab is part of a NeuroNex Network with the goal of addressing the foundational question: How do biological nervous systems control and execute interactions with the environment?
Our network, which includes scientists and engineers from ten institutions across the United States, the United Kingdom and Germany, is focusing on Communication, Coordination, and Control in Neuromechanical Systems (C3NS) to develop comprehensive models of sensorimotor control with relationships to the environment, both within individual species, and across the phyla Arthropoda, Mollusca and Chordata.
Together, we seek to create a conceptual modeling framework that can predict control for organisms of different size and speed scales. Through our inter-phylum experimental study of sensorimotor control, we seek to identify convergent or conserved principles to refine and inform this framework. Such a framework will have a tremendous effect on the ability to interpret, and extend the impact of, experimental results across biology and robotics, with future applications to prosthetics.
The Ache Lab will closely collaborate with the Büschges, Ito and Blanke Labs at the University of Cologne and Nick Szczecinski’s Lab at the University of West Virginia to contribute a model of Drosophila motor control to the project. C3NS is led by Roger Quinn at Case Western Reserve University.
We are working together with the Frye Lab at UCLA to push our understanding of olfactory modulation of visual sensorimotor circuits using 2-P imaging tools and techniques.
Büschges Lab (University of Cologne)
We are closely collaborating with the Büschges Lab on our C3NS NeuroNex project studying the descending control of locomotion. Currently, Michael Dübbert and Till Bockemühl are helping us put together the latest generation of fly virtual reality arenas (with help from Michael Reiser at Janelia). We are also working on a secret mission to have at least 50% of the Büschges Lab moving to Würzburg by 2022.
Haluk Lacin, PhD (Washington University in St. Louis)
We are collaborating with Haluk on a project that involves chewing lots of food (preferably Iskender).
Carlos Ribeiro, PhD (Champalimaud Centre for the Unknown)
Carlos and his team are experts in nutrition, and we are collaborating on a project that aims to resolve how sensory inputs converge onto modulatory networks that set and respond to metabolic state changes. The Ribeiro lab is developing and running nifty behavioral experiments for this project, which, combined with our circuits work, help us understand the circuits underlying food-sensing.
Prof. Sabine Fischer (CCTB, Würzburg) and Lorenzo Fontolan, PhD (HHMI, Janelia Research Campus)
Sabine and Lorenzo are modeling experts and enthusiasts, who are helping us build theoretical models of the modulatory circuits we are characterizing electrophysiologically.
Prof. Tanja Godenschwege (Florida Atlantic University)
Tanja is a visiting scientist with the lab and together we are working on some nifty molecular signaling pathways.
Prof. Mark Frye (UCLA)
The Frye and Ache labs share an interest in modulation of visual sensorimotor pathways, and we’ve recently acquired funding from BaCaTec to help kickstart our joint foray into that world.
Gwyneth M. Card, PhD (HHMI, Janelia Research Campus
We are working on characterizing the networks underlying landing responses in Drosophila with Jan’s postdoc advisor, Gwyneth Card. This project is supported by a Janelia visitor project grant.
Locomotion needs to be adjusted to ever-changing environmental and internal demands. For example, animals need to respond to obstacles or conspecifics in their path and integrate a range of sensory information to navigate them, for instance by walking around the obstacle or waiting for the conspecific to pass. To this end, information from diverse and distinct sensory modalities, such as vision or mechanosensation, is integrated by brain circuits, and converges onto a relatively small population of descending neurons. These “output neurons” of the brain communicate with motor centers in the ventral nerve cord, where they control various behaviors, such as curve walking or stopping, an thus adapt motor programs to cope with changes in the environment. To deliver insights into fundamental principles facilitating this sensorimotor flexibility, Sander is combining various techniques such as whole-cell patch clamp recordings, optogenetics, and behavioral analysis.
After finishing his Bachelor’s thesis at the Max Planck Institute for Neurological Research, Sander pursued his postgraduate studies at the University of Cologne where he qualified for the Fast-Track Masters/Doctoral program. He earned his PhD in neurobiology in Ansgar Büschges’ and Reinhard Predel’s Labs. His thesis focused on the identification and role of neuropeptides in insect motor control.
Rituja has always been intrigued by the complexity of the brain: How does the state of mind affect our behavior? What are the neural mechanisms underlying decision making? What is the role of the environment in shaping our behavior? To answer some of these questions in her PhD project, Rituja is investigating how sensory inputs and internal states are integrated to shape the activity of neuronal networks in the Drosophila brain. Currently, she is spending most of her time befriending fruit flies and convincing them that it makes absolute sense to expose their brains to her, so that she can access their neurons and perform patch-clamp recordings.
Rituja holds a B.Sc in Biochemistry, Genetics and Biotechnology from Bangalore University, India and an MRes in Tissue Engineering and Regenerative Medicine from the University of Manchester, UK. Later, she moved to Germany to pursue her postgraduate studies and obtained a Masters in Molecular and Developmental Stem Cell Biology from Ruhr University Bochum. She carried out her master‘s thesis titled, “Effect of BAP1 mutations on DNA methylation pattern in human iPSCs” in Dr. Steenpaß’s lab at the Institute of Human Genetics, Essen.
To ensure survival in an ever-changing, complex world, animal behavior needs to be flexible and adaptive. Nervous systems have evolved to enable behavioral responses to a wide variety of sensory stimuli, but the adequate behavioral response to a given stimulus is highly context-dependent, and behavioral or internal states accordingly affect sensorimotor processing. For example, locomotion modulates responses of visual neurons, and hunger increases food-searching behavior and shifts taste preferences. Despite their ubiquitous importance, the neural mechanisms enabling context-dependent sensorimotor flexibility are not well understood. The Ache Lab for Sensorimotor Flexibility aims to discover fundamental principles of motor control, in particular with regard to sensorimotor flexibility, by leveraging the power of neurogenetics, electron microscopy-based circuit reconstruction, and in-vivo patch-clamp recordings in behaving Drosophila.